Log in
BACKGROUND:
Diabetes mellitus alters oxidative stability and immune response. Here, we investigated the impact of a peptide extracted from camel milk (CMP) on the oxidative status, transcription factor kappa - B (NF - kB) and inflammatory cytokine in diabetic wounds.
METHODS:
Rats were assigned into three groups: control, diabetic induced (DM) and diabetic induced with multiple doses of CMP for a week (DM - CMP).
RESULTS:
DM showed a sharp decline in the activity of major antioxidant enzymes such as supe roxide dismutase (SOD), catalase (CAT) and glutathione (GSH) compared to the control. The DM - CMP group, however, showed a noticeable replenishment in the activity of these enzymes compared to the DM group. The CMP - treated group also showed a normal level o f lipid peroxidation marker (MDA) compared to the DM rats. Furthermore, ELISA analysis of serum TNF - α protein showed an elevated level in diabetic rats in comparison to control serum. However, RT - PCR analysis of locally wounded skin tissues revealed that d iabetes down - regulates the RNA expression of both TNF - α and MIF genes in comparison to the control samples but that CMP was found to restore RNA expression significantly. Although it was elevated in CMP - treated rats after one day of wound incision, the NF - kB protein level was significantly decreased seven days after the incision in comparison to the animals in the diabetic group.
CONCLUSIONS:
CMP, therefore, can be seen an effective antioxidant and immune stimulant that induces oxidative stability and speeds up wound healing in diabetic model animals, making it a potential adjuvant in improving wound healing in those with diabetic conditions.